You have loaded in your gel a protein sample called IG Boil, which contains a protein mixture that includes GFP and many other proteins...

I am almost positive it is multiple bands i just dont know how to explain why can someone explain. And please do correct me if i am wrong.

3. You have loaded in your gel a protein sample called "IG Boil", which contains a protein mixture thatincludes GFP and many other proteins of different sizes. In last week’s lab, this was the protein mixture you originally loaded into the column. Recall that this sample was treated with denaturingsolution and boiled before it was loaded in the gel. After electrophoresis and Coomassie Blue staining, would you expect to see a single protein band ormultiple bands in the lane for this sample? Briefly explain your reasoning.