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QUESTION

You're trying to PCR amplify a gene, but gel shows that there are two bands instead of only one like you expected.

You're trying to PCR amplify a gene, but gel shows that there are two bands instead of only one like you expected. You know for certain that there are no homologous genes in the genome from which you're amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.

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