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10.(Bonus: 2 pts extra credit) A graduate student wanted to identify the enzymes involved in cell division for a bacillus-shaped bacterium whose genome lacked many cell division genes found other species. Since cell division is an essential process, she had to create conditional mutants. She first transformed the cells with a transposon that will insert a xylose-inducible promoter into the genome at random sites, with each cell likely to have a single insertion event.
She then plated her collection of mutagenized cells onto media containing the growth- permissive media additive, grew up colonies, and replica plated the colonies from each of these master plates onto two sets of plates: media containing glucose and media containing xylose.
Next, she identified colonies with a defect in cell division that were smaller or absent from the non-permissive plate compared with their growth on the permissive plate.
Finally, she grew up individual mutant colonies in permissive media, transferred the cultured cells into fresh, non-permissive media, and viewed them by microscopy to confirm their cell division defect.
Question: On which plate would she be able to observe the defective colonies [Glucose or Xylose] and what would the phenotype of the defective cells be when viewed by microscopy? Explain your answer.
