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An enzyme, alphabetase, catalyzes this reaction: C---D. Researchers find that the the K m for the substrate C is 7uM, and the K cat is 800 s -1 .

An enzyme, alphabetase, catalyzes this reaction: C<--->D. Researchers find that the the Km for the substrate C is 7uM, and the Kcat is 800 s-1. The compound Y is found to be a very strong competitive inhibitor of the enzyme, with an  of 5. In an experiment with the [E]T = 20uM, an amount of Y is added that reduces V0 to 8,000nM/s. What is [C] in this experiment? Recall that Kmapp = αKm for a competitive inhibitor. How can you determine [C] with minimal calculation?

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