An enzyme was isolated from wild-type bacteria (WT). Three mutant forms of this same enzyme were made using a mutagenesis technique, and then each mutant enzyme (1E8, 5F8, D249G) was isolated. All fou

An enzyme was isolated from wild-type bacteria (WT). Three mutant forms of this same enzyme were made using a mutagenesis technique, and then each mutant enzyme (1E8, 5F8, D249G) was isolated. All four samples of purified enzymes were tested for thermal stability. To do this, each enzyme was incubated at the temperatures indicated on the graph for 15 minutes and then tested for the percentage of original catalytic activity that remained after the heat treatment. Describe the effects of the mutations on the thermal stability of the wild-type enzyme. Analyze the data to rank the four in order from least heat-stable to most heat-stable.