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B Brock University - Welcome to Br X B Brock Sakai : BIOL 1P92 LAB Wint X B Microsoft Word - Experiment 2 Li X B Halophilic Archaea * Desired...
I have to answer these two questions questions #8 and #9 on page 8
8. CONCLUSIONS: Based on the differentiating morphological, physiological, biochemical, and phylogenetic characteristics, make brief statements justifying the proposal for assigning your new haloarchaeal species to a new genus or to an existing one. This is your final chance to reinforce the importance of your findings to your reader.
9. DESCRIPTION PARAGRAPH: Often, authors provide a paragraph describing the characteristics of their newly discovered isolate. When describing your halophile, follow the format of the example. Remember to include the results of all the tests you performed. Also indicate if your halophile is (1) photoautotrophic, chemoautotrophic, photoheterotrophic or chemoheterotrophic; (2) an obligate aerobe, facultative anaerobe, or a strict anaerobe; (3) psychrophilic, mesophilic, or thermophilic and (4) acidophilic, neutrophilic, or alkaphilic. Also summarize how the name of your isolate was derived. Give your isolate a strain an alphanumeric designation. Example: Description of Haloterrigena japonicum strain A7 Haloterrigena (Greek noun, hal/halo salt; Latin adjective, terrigena of the earth) japonicum (Latin adjective japonicum pertaining to Japan) Colonies on solid agar medium are translucent, have a smooth, shiny surface and are light red. They appear convex with an entire margin. Colonies are circular and are about 1-2 mm in diameter. Cells are non-motile and do not produce gas vesicles. Growth occurs at NaCl concentrations of 2.2-4.0 M (optimum at 3.5 M), at pH 6.5-8.0 (optimum at pH 7.0) and at 37-52oC (optimum at 40oC). Chemo-organotrophic and an obligate aerobe. Oxidase- and catalase-positive. Indole formation from tryptophan is positive. Reduces nitrate to nitrite. Gelatin, starch and Tween 20 are not hydrolysed. Casein is hydrolyzed. Hydrogen sulfide is produced from thiosulfate. Acid produced from glucose, galactose and sucrose but not maltose. Cells are resistant to ampicillin, novobiocin and penicillin but sensitive to bacitracin.
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