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Determine the structural nature of this protein: Prelim MW determination- gel filtration: 1 peak that elutes half way between myosin and...
Determine the structural nature of this protein:
- Prelim MW determination- gel filtration: 1 peak that elutes half way between myosin and B-galactosdase (which is about 158,125Da)
- Gel electrophoresis: results are not interpretable
- Subunit makeup via gel filtration: 1 peak that elutes about 7% slower than ovalbumin
- subunit makeup via SDS PAGE: 1 peak that elutes about 7% faster than ovalbumin
- disulfide bonds via gel filtration: 2 peaks, one that elutes with carbonic anhydrase and one that elutes after lysozyme
- disulfide bonds via SDS PAGE: 2 peaks, one that migrates the same as carbonic anhydrase and one that migrates faster than lysozyme.