Exactly 5.00 mL aliquots of a solution containing Phenobarbital were measured into 50.00ml volumetric flasks and made basic with KOH.

Exactly 5.00 mL aliquots of a solution containing Phenobarbital were measured into 50.00ml volumetric flasks and made basic with KOH. The following volumes of a standard solution of Phenobarbital containing 2.00 µg/ml of Phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.00, 0.500,1.000, 1.50, and 2.00ml. The fluorescence of each of these solutions was measured with a flurometer, which gave volumes of 3.26, 4.80, 6.41, 8.02, and 9.56 respectively.a) Pot the data?b) Using the plot from (a) calculate the concentration of Phenobarbital in the unknown.c) Derive a least- squares equation for the data.d) Find the concentration of Phenobarbital from the equation in (c)e) Calculate a standard deviation for the concentration obtained in (d)Chemistry