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Explain why quantum yield and the extinction coefficient are important factors to consider when using a fluorescent based method. What are the

  1. Explain why quantum yield and the extinction coefficient are important factors to consider when using a fluorescent based method.
  2. What are the consequences if your drug binds too tightly to HSA? What if it doesn't bind to HSA at all? How might the binding affect the efficacy of your drug?
  3. When choosing a fluorescent probe for an experiment like this lab, what are two things you would want to consider?
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