Answered You can hire a professional tutor to get the answer.
Figure 5. Effects of Molecule X on casein processing by macrophages.BMDMs
4:03 11 4GC. .StdsPBS Chlq.Molecule XScrambled20505 20 505202605080FITCdetection2015- 1108060Loadingcontrol50(total protein)40Obtained gels were then scanned in the FITCchannel to determine the density of thedetected bands. Under optimal processingconditions, digested casein loses some of itsability to fluoresce, andthe emitted signal can be used as a referencevalue (i.e. corresponding to the total signalemitted after normal processing of casein bymacrophages) for analyses of the effectsof specific sample treatments. If processingis enhanced, then the fluorescence emittedby the digested casein will be lower than thatof the normal processing control. On theother hand, if processingis impaired, undigested casein will be able toemit a higher FITC fluorescing signal thanthat of the normal processing control (inother words, fluorescence intensity inverselycorrelates to the antigen processing ability ofmacrophages).Figure 5. Effects of Molecule X on caseinprocessing by macrophages. BMDMs wereincubated for 3h with FITC-Casein with orwithout PBS, chloroquine (Chlq. at 20 and50 uM), molecule X orscrambled molecule at increasingconcentrations (5, 20, and 50 ug/mL).Sample lysates and molecular weightstandards (Stds. ) were run by CDS DAGD
