Here are the questions which is about the paper of "A Virus in a Fungus in a Plant:Three-Way Symbiosis Required for Thermal Tolerance". I attached the file and I wish I can get the correct answer for

Here are the questions which is about the paper of "A Virus in a Fungus in a Plant:Three-Way Symbiosis Required for Thermal Tolerance". I attached the file and I wish I can get the correct answer for that. Thanks.

1) What does it mean for a virus to 'attenuate' the 'virulence' of a fungus on a plant? Define both terms in apostrophe and give example from paper.

2) Describe the process of 1) reverse transcription, 2) amplification, and 3) cloning in your own words.

3) What does it mean for a gene sequence to be described as 'putative'?

4) What does it mean for a virus to be transmitted 'vertically'? What would be the opposite of 'vertical transmission'?

5) Why did authors use simple sequence repeat analysis and sequence the rDNA region of the fungal isolate with low titer of the virus? What was the purpose of these tests?

6) Why did authors reintroduce the virus to the virus-free fungal isolate? If the fungus with reintroduced virus did not confer heat-tolerance, what would the authors have concluded?