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QUESTION

# How will you prepare a 1:80 dilution?

The answer Rogan wrote is correct, but his example is a little off.

Adding 80 mL of water to 1 mL of stock solution will produce a 1:81 , not a 1:80 one.

The ratio between the final volume of the solution, the one you get after diluting the initial sample, and the initial volume of the sample will give you the dilution factor So,

"DF" = V_"final"/V_"initial"

If you've got a 1-mL sample, and the dilution factor is 1:80, you'd get

V_"final" = "DF" * V_"aliquot" = 80 * 1 = "80 mL"

However, this volume includes the volume of the aliquot, the original sample you wanted to dilute, which means that the 80-mL final volume will include 1 mL of sample, the rest being the volume of the diluent

V_"diluent" = V_"final" - V_"aliquot" = 80 - 1 = "79 mL"

So, to make a 1:80 dilution for a 1-mL sample, you add enough water to get the final volume to 80 mL -> in this example, you would add 79 mL of water to the 1-mL sample.

If you add 80 mL of water, you'll get a dilution of 1:81

"DF" = (80 + 1)/1 = 81