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Looks like we are all out of Basic Germination Media!!! How will be able to perform hanging drop experiments

Looks like we are all out of Basic Germination Media!!! How will be able to perform hanging drop experiments

now?

Luckily we know what Basic Germination Media consists of; we know it is made up of 1mM KCl, 0.1mM CaCl2, 1.6mM H3BO3, and 10% Glucose. We also have stock solution of each component consisting of 150mM KCl, 100mM CaCl2, 20mM H3BO3, and 20% Glucose solution. 

How much of each solution will you need to make 10mL of Basic Germination Media?

Make sure to include the units

KCl  ?

CaCl2  ?

H3BO3 ?

Glucose  ?

Water  ?

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