MIC 140 Bacterial Physiology NAME_______________________________ Problem Set #2 REQUIRED Due October 12, 2011 1.

MIC 140 Bacterial Physiology NAME_______________________________Problem Set #2 REQUIRED Due October 12, 20111. Calculate the energy cost to make 1 gram of Escherichia coli, assuming in this case that E. coli is an autotroph using the Reductive Pentose Phosphate cycle (Calvin, or Calvin, Benson, Bassham cycle) to assimilate CO2 into biomass; see Fig. 13.3 in the textbook. To solve this problem you need to calculate the ATP cost in making the 11 (12) precursor compounds at the cellular concentrations required, based on the reactions of the Reductive Pentose Phosphate cycle. Record your results in a Table format as was done in the class handout when glucose or malate served as the carbon source. Then in a second table, add up the total amounts of ATP required for precursor, monomer (building block) and polymer (macromolecule) synthesis and compare to E. coli growing as an organoheterotroph on glucose. You need not include reducing power (NADH and NADPH) or carbon costs.