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QUESTION
One litre of [3H]uridine with a concentration of 100 mmol cm3 and 50 000 c. cm3 is required.
- One litre of [3H]uridine with a concentration of 100 mmol cm3 and 50 000 c.p.m. cm3 is required. If all measurements are made on a scintillation counter with an efficiency of 40%, how would you make up this solution if the purchased supply of [3H]uridine has a radioactive concentration of 1 mCi cm3 and a specific activity of 20 Ci mol1, 0.75 TBqmol1? [NB: Mr uridine¼ 244; 1 Ci¼22.2 1011 d.p.m.]
