P7.16: 2-amino-2-hydroxymethylpropane-1,3-diol, (commonly known as

Chapter 7: Acid-base reactionsP7.16: 2-amino-2-hydroxymethylpropane-1,3-diol, (commonly known as 'Tris') andimidazole are very commonly used as buffers in biochemistry and molecular biologylaboratories. You make two buffer solutions: One is 50 mM Tris at pH 7.0, the other50 mM imidazole/imidazolium at pH 7.0 For each solution, calculate the concentrationof buffer molecules in the cationic protonation state.OHHNoHaN-COHNOHH'Tris' bufferimidazoliumPK, = 8.1PK =7.0