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Question 1 Mismatch repair requires ___ enzymatic activity. Endonuclease DNA Polymerase Ligase All of the above 0.
Question 1
- Mismatch repair requires ___ enzymatic activity.
- Endonuclease
- DNA Polymerase
- Ligase
- All of the above
0.5 points
Question 2- Pyrimidine dimers that are detected by the cell are most likely to be repaired using:
- Mismatch repair
- Proofreading
- Excision repair
0.5 points
Question 3- Conversion of C to G in DNA is an example of a:
- Tautomerization
- Transversion
- Transition
- Pyrimidine dimer
0.5 points
Question 4- Shown below is a diagram of a eukaryotic replication bubble with only the template shown. Where on the diagram is the leading strand? (Examine the template DNA very carefully before answering this question!)
- A
- B
- C
- D
0.5 points
Question 5- Shown below is a diagram of a eukaryotic replication bubble with only the template shown. Where is the lagging strand? (Examine the template DNA very carefully before answering this question!)
- A
- B
- C
- D
0.5 points
Question 6- Shown below is a diagram of a eukaryotic replication bubble with only the template shown. Where is the lagging strand? (Examine the template DNA very carefully before answering this question!)
- A
- B
- C
- D
0.5 points
Question 7- What problem is caused by the formation of Okazaki fragments and what is the cell's solution?
- They result in excessive twisting of the DNA, which is relieved by Topoisomerase.
- They are short, discontinuous stretches of DNA, which must be annealed using Ligase.
- They require DNA synthesis to occur in the 3' to 5' direction, which is catalyzed by Primase.
0.5 points
Question 8- What kind of enzyme catalyzes polymerization of DNA?
- Endonuclease
- Polymerase
- Primase
- Helicase
0.5 points
Question 9- What problem associated with DNA replication is solved by the formation of Okazaki fragments?
- DNA replication begins at multiple origins of replication in eukaryotes.
- The two strands of DNA are held together by millions of hydrogen bonds, making separation extremely difficult.
- DNA must polymerize in the 5' to 3' direction on a template.
- DNA polymerization requires an existing chain onto which to add new dNTPs.
0.5 points
Question 10- What would you observe in a cell in which DNA helicase was inactivated?
- DNA replication would fail due to the inability to separate the two strands of DNA.
- DNA replication would fail due to the absence of a primer for DNA polymerization.
- DNA replication would begin, but would eventually stall due to the increase in twisting beyond the replication fork.
- DNA replication would occur on the leading strand, but not the lagging strand.