Show data you used in your actual calculations (e.

Hi, I need help on calculating the final concentrations for both Chl a and Chl b. Show data you used in your actual calculations (e.g. fresh weight of the tissue, volume of acetone added, 80% acetone used for dilution, OD645, OD663, etc). Pay attention to the unit you will use to express your final chlorophyll a and chlorophyll b concentrations.

1) 80 % acetone 2) 1g = 1ml of fresh weight 3) dilution OD645, OD663,

Results A= 0.11 T= 77 @ 645 nm A= 0.19 T=65 @ 665

Calculate chorophyl/ a and chorophyll b

A645= (16.75ml/mg chla cm)[chla] (ln)+ (45.6 ml/mg chlb cm) [chlb] (ln)

A663 = (82.04ml/mg chla cm)[chla] (ln)+ (9.27 ml/mg chlb cm) [chlb] (ln)