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Two portions of the dna were individually digested and a third portion of the dna sample is double digested with both restriction enzymes. after running it through the gel electrophoresis, the size of
Two portions of the dna were individually digested and a third portion of the dna sample is double digested with both restriction enzymes. after running it through the gel electrophoresis, the size of the dna fragments are recorded. the total length of the fragments in each digestion should be equal, but the length of each individual dna fragment depends on where its respective restriction site is located. each restriction site can be mapped according to the lengths of the fragments, and the information from the double digest is crucial in correctly mapping the sites. the drawing of the dna segment displays where the restriction sites are located, and this drawing is referred to as a restriction map.
