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1.      Using the data below:

Average Fruit Production/Plant

Hormone Level (mg/L)

4.0

0.0

4.1

10.0

13.2

20.0

17.0

30.0

25.6

40.0

33.5

50.0

37.3

60.0

a.      (5 points) Make a standard curve using MS Excel (using a linear trendline). Make sure to display the equation of the trendline on your graph. Attach your graph.

b.      (3 points) Using your standard curve, determine how much fruit a plant will produce if it is injected with 43 mg/L of hormone.

2.      Using MS Excel, prepare a standard curve (using a logarithmic trendline) from the data below:

Molecular Weight Marker (bp)

Distance Migrated (mm)

2000

4

1500

5

1000

7.5

750

10

500

14

300

21

150

30.5

a.      (5 points) Display the equation of your trendline on the graph and attach your graph.

b.      (3 points) Using your standard curve, determine the size (in bp) of a DNA fragment with a migration distance of 17.5 mm. 

3.      Cells in culture are treated in such as way that they are expected to take up a fragment of DNA containing a gene that codes for an enzyme. The activity of the enzyme in cells that take up the gene can be assayed. The more active the enzyme, the better. Suppose a researcher isolates 45 clones of cells and measures the enzyme activity in each clone. The results are shown below in activity units

10.4

14.2

13.0

9.0

10.1

9.8

8.9

3.2

12.0

10.2

10.1

0.4

2.3

9.1

9.2

11.0

9.1

12.1

8.9

5.6

12.0

7.8

11.3

8.6

13.6

12.2

15.1

9.8

2.1

10.2

10.1

9.4

9.8

12.3

2.1

12.3

10.6

10.8

12.4

12.0

13.1

5.8

12.8

12.3

1.5

a.      (5 points) Using MS Excel, find the minimum, the maximum, the median, the mean (average), and the standard deviation for the enzyme activity data from the isolated clones.

Minimum

Maximum

Median

Mean

Standard Deviation

b.      (4 points) Using MS Excel, make a histogram graph of the data. Format the horizontal axis and set the bin width to 1.0. Indicate on the plot where the mean and median are located.

A solution is made by dissolving 3.88 g of KCl in enough water to make 67.8 mL of solution.  What is the concentration of potassium chloride in units of weight/volume percent?

0.0572% (w/v) KCl , 5.72%(w/v) KCL , 5.7%(w/v)  KCL, 6%(w/v)KCL

A solution is made by mixing 569 mL of water with 238 mL of 100% (v/v) ethanol.  What is the concentration of ethanol in units of volume/volume percent?

0.418%(V/V) ethanol , 29.2%(v/v) ethanol , 0.295% (v/v) , 41.8% (v/v) ethanol

Your lab mate is planning an animal experiment in which she needs to deliver 7.7 mg of the drug methotrexate to each control animal.  She's purchased a solution of methotrexate at a concentration of 0.92% (w/v).  What volume of the methotrexate solution must be administered to each animal?

837 μL methotrexate solution

7.1 mL methotrexate solution

8.4 mL methotrexate solution

119 μL methotrexate solution

The density of glycerol is 1.26 g/mL.  What is the mass of 18.0 mL of glycerol?

Group of answer choices

18.2 g

22.7 g

14.2 g

30 g

In order to distinguish bacteria containing the pGREEN plasmid from those that don't, ampicillin is added to the broth.  If you pipetted 40 μL from an ampicillin stock tube of concentration 10 mg/mL, how much ampicillin did you remove from the stock tube?

Group of answer choices

400 μg

400 mg

100 μg

100 μg/mL

A standard polymerase chain reaction (PCR) experiment uses 50 ng of plasmid DNA per tube.  If your plasmid purification activity from lab yielded 7.6 μg/mL of pGREEN DNA, what volume of your pGREEN plasmid will you add to your PCR reaction tube?

Group of answer choices

380 μL

6.5 mL

6.6 μL

4 mL

How many micromoles of solute are present in 250 mL of a 0.10 mM solution?

Group of answer choices

4.0 μmoles

0.025 μmoles

25 μmoles

0.4 μmoles

A particular solution requires 150 mg of enzyme per mL of solution to be useful.  How much enzyme is needed to make 1.0 x 104 μL of solution?

Group of answer choices

1500 mg

150 mg

15 mg

150 mL

If there are about 2.0 x 102 blood cells in a 1.0 x 10-2 mL sample, how many blood cells would be in 2.2 mL of this blood?

Group of answer choices

40,000 cells

4400 cells

44,000 cells

4000 cells

If 20.0 g of DIFCO LB powder is needed to make 1.2 L of microbiological medium, how much medium can be made using 13.1 mg of DIFCO LB powder?

Group of answer choices

0.000786 L

786 mL

7.90 mL

790 μL

If the conversion factor between absorbance readings at 260 nm and DNA concentration is 1.00 A.U. = 50.0 μg/mL, and you have measured a sample of your DNA to have 0.540 A.U., how much mass of DNA do you have if your total volume of DNA is 50 μL?

Group of answer choices

27 μg

270 ng

1.35 μg

1350 μg

You have collected the following data regarding species of trees on the NOVA-Manassas Campus.  There might be more species of trees and higher numbers, but you only focused on one area of the campus.

Species of Tree

Number observed

Beech

54

Oak

125

Pine

32

Which graph would be the best one to use to display your data?

Group of answer choices

A Bar Chart

A Line Graph

A Scatterplot

A Pie Chart

Using the following standard curve, calculate the concentration of an unknown protein sample with an absorbance value of 0.754

You have measured the concentration of your DNA sample to be 57.4 ug/mL. What is your DNA concentration in ng/uL units?

Group of answer choices

57400 ng/uL

574 ng/uL

5.74 ng/uL

57.4 ng/uL

A 50.0 mM stock solution of MgCl2 is commonly used in labs for polymerase chain reaction (PCR) experiments.  How much MgCl2 would you weigh out to make 550 mL of a 50.0 mM MgCl2 stock solution? (FW for MgCl2 = 95.21 g/mol)

Group of answer choices

26 mg

26 g

2.6 g

275 mg

The following data have been collected over the past several years for the NOVA-Manassas Campus.

Year

Number of Full-Time Equivalent Students

2008

2776

2009

3086

2010

3307

2011

3428

2012

3637

2013

3884

2014

3998

2015

4217

2016

4265

2017

4316

Which graph would be the best one to use to display this data?

Group of answer choices

Line Graph

Pie Chart

Histogram

Bar Graph

s

List three things that are wrong about the graph below: