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You add a nuclear localization sequence at the Cterminus of GFP and a nuclear export sequence at the Nterminus.
Q:
You add a nuclear localization sequence at the Cterminus of GFP and a nuclear export sequence at the Nterminus. When you express this protein, 70% of it is located in the nucleus and the rest is present in the cytoplasm. You assume that this protein is shuttling between the nucleus and the cytoplasm. If you add an inhibitor of nuclear export to these cells what happens to the distribution of this protein? (5 points)A. No difference in the distributionB. All protein will be in the cytoplasmC. Protein will still be shuttling between nucleus and cytoplasmD. All protein will be in the nucleus E. Increased accumulation in the cytoplasm
