Your colleague gives you data collected for the rate of product formation from enzyme E and substrate S.

Your colleague gives you data collected for the rate of product formation from enzyme E and substrate S. You are asked to propose a mechanism for the enzyme substrate reaction so that the product formation can be predicted in the future (rather than having to collect the data each time. Your overall reaction is of the form E + S -> E + P. You start by assuming that the enzyme substrate complex is of the form ES and that this is a reactive intermediate. Your revised reaction equation is then: E + S <-> ES -> E + P. You then try to predict product formation using the quasi-steady state assumption for ES. You find that this assumption doesn't match the data in terms of estimated rate of product formation. You then try the rapid-equilibrium assumption for ES. Once again, the resulting expression for the rate of product formation does not match the collected data. Describe two possibilities that would you test next to determine the reaction mechanism.