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QUESTION

Your work at DuPunt, Inc. continues with the microbe ETPUM, but you have been shifted to the Division of Disposable Diapers.

Your work at DuPunt, Inc. continues with the microbe ETPUM, but you have been shifted to the Division of Disposable Diapers. The chemical engineers have devised two different super-absorbent polymers [poly-α-ketoglutarate (PK) and poly-fumarate (PF)]. Both polymers have done wonderfully in their "field tests" (Thank goodness you don't have that job!). Now the engineers would like to ensure that these polymers are biodegradable, and they have asked for your advice on which polymer (poly-α-ketoglutarate or poly-fumarate) would be better to use from this perspective. Both polymers have molecular weights > 1,000,000 Daltons. The engineers would like to be able to be able to add ETPUM to a landfill full of DuPunt Disposable Diapers, and then let the microbes break down the absorbent polymer), converting it into organic molecules. Note: ETPUM would need to be able to grow using the polymer in the diapers as a carbon and energy source.Your recent work with ETPUM indicates that it:● secretes an enzyme (“Diaperase”) that digests PK (generating α-ketoglutarate ) and PF (generating fumarate)● has a PMF-powered transporter that can transport either α-ketoglutarate or fumarate across the cytoplasmic membrane (at a cost of 10 protons per molecule of α-ketoglutarate or fumarate transported)● has all of the enzymes required for glycolysis and the TCA cycle, an ATP synthase and an Electron Transport Chain that uses O2 as terminal electron acceptor (assume that these work just in Bauman Fig. 5.13, 5.16, and 5.18).Answer the specific questions (2-5) listed below; then come back and answer the overall question (#1):1. Overall Question: Which would be better to use in the diapers? PK or PF?

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