Waiting for answer This question has not been answered yet. You can hire a professional tutor to get the answer.
1. If the kcat for enolase in E. coli is 13 200/min, what would be the Vmax value if 1.92 uM enolase was present in the cell in mM/sec?
1. If the kcat for enolase in E. coli is 13 200/min, what would be the Vmax value if 1.92 uM enolase was present in the cell in mM/sec?
2. If the concentration of 2-phosphoglycerate is 1/30 of the Km value of enolase (2.62 uM), what would be the rate of the reaction?
3.
[S] Vo Vo Vo
0.33 1.65 1.05 0.79
0.5 2.13 1.43 1.02
1 2.99 2.22 1.43
2 3.72 3.08 1.79
5 4 3.8 2
a) Generate a single L-B plot for all the enzymatic reactions.
b) Using the L-B plot you created in a), determine the Km and Vmax for all the reactions.
c) Determine the type of inhibition for both inhibitors B and C. Explain how you arrived at your conclusions. (2 marks)
